golden gate enzyme mix (New England Biolabs)
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99
Structured Review
New England Biolabs
golden gate enzyme mix
Golden Gate Enzyme Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 321 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/golden+gate+enzyme+mix/NEBridge+Golden+Gate+Assembly+Kit+(BsaI/bio_rxiv__64898__2026__04__21__719857-273-23-28
Average 99 stars, based on 321 article reviews
Golden Gate Enzyme Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 321 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/golden+gate+enzyme+mix/NEBridge+Golden+Gate+Assembly+Kit+(BsaI/bio_rxiv__64898__2026__04__21__719857-273-23-28
Average 99 stars, based on 321 article reviews
golden gate enzyme mix - by Bioz Stars,
2026-09
99/100 stars
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other:Article Title: Systematic genome-wide discovery of host factors governing bacteriophage infectivity Article Snippet: This reaction included mixing 75 ng of linearized psgRNA01 backbone, 1 μl of diluted dsDNA oligo, 2 μl of T4 ligase buffer, 1 μl of Article Title: Standardized Iterative Genome Editing Method for Escherichia coli Based on CRISPR-Cas9. Article Snippet: The introduction of complex biosynthetic pathways into the hosts’ chromosomes is gaining attention with the development of synthetic biology.. While CRISPR-Cas9 has been widely employed for gene knock-in, the process of multigene insertion remains cumbersome due to laborious and empirical gene cloning procedures.. To address this, we devised a standardized iterative genome editing system for Escherichia coli, harnessing the power of CRISPR-Cas9 and MetClo assembly. Article Title: Systems and methods for fully automated protein engineering Article Snippet: Per 10 composed of 7 μL water, 2 μL Article Title: Systematic genome-wide mapping of host determinants of bacteriophage infectivity Article Snippet: This reaction included mixing 75 ng of linearized psgRNA01 backbone, 1 μl of diluted dsDNA oligo, 2 μl of T4 ligase buffer, 1 μl of Article Title: Rapid Cell-Free Combinatorial Mutagenesis Workflow Using Small Oligos Suitable for High-Iteration, Active Learning-Guided Protein Engineering Article Snippet: The resulting DNA mix was used to setup a Golden Gate assembly by preparing a solution of: 58.33% DNA mix, 26.67% nuclease-free water, 10% Plasmid Preparation:Article Title: Landscape-scale navigation unlocks antibody CDR structural logic for AI-guided rescue and therapeutic optimization Article Snippet: The digested vectors and the annealed dsDNAs were mixed at a molar ratio of 1:30, and ligated using T4 DNA ligases (M0202; New England Biolabs) in T4 DNA Ligase Reaction Buffer (B0202; New England Biolabs) for 2 hours at 16 °C. .. For multiple CDR substitutions, 400ng of modular vector was combined with equimolar amounts of dsDNA fragments encoding CDR variants and framework regions using |